quant ittm picogreen double stranded dna assay kit  (Thermo Fisher)

Journal: Zygote

Article Title: A chemical compound based on methylxanthine–polyphenols lowers nitric oxide levels and increases post-thaw human sperm viability

doi: 10.1017/s0967199417000600

Figure Lengend Snippet: Figure 2 Pre-freezing analyses of sperm exposed to L-CCMP, I-CCMP or H-CCMP. (a) Cell viability was evaluated by flow cytometry using propidium iodide (PI) staining. (b) Cell viability was evaluated using crystal violet assay. (c) cfDNA was measured by PicoGreen® assay. The results were compared with the control (cells and medium only). N = 3, ∗∗∗P < 0.0001.

Article Snippet: The Quant-ITTM PicoGreen® double-stranded DNA kit (Invitrogen, Life Technologies) was used to perform cfDNA determination.

Techniques: Flow Cytometry, Staining, Crystal Violet Assay, Picogreen Assay, Control

Journal: Zygote

Article Title: A chemical compound based on methylxanthine–polyphenols lowers nitric oxide levels and increases post-thaw human sperm viability

doi: 10.1017/s0967199417000600

Figure Lengend Snippet: Figure 4 Post-thaw analyses of sperm exposed to L-CCMP, I-CCMP or H-CCMP and storage at liquid nitrogen. (a) Cell viability was measured by flow cytometry using PI. (b) Levels of viable cells were analyzed by crystal violet assay. (c) cfDNA levels were evaluated by PicoGreen assay. The results were compared with the control (cells and medium only). N = 3, ∗P < 0.005.

Article Snippet: The Quant-ITTM PicoGreen® double-stranded DNA kit (Invitrogen, Life Technologies) was used to perform cfDNA determination.

Techniques: Flow Cytometry, Crystal Violet Assay, Picogreen Assay, Control

Journal: Zygote

Article Title: A chemical compound based on methylxanthine–polyphenols lowers nitric oxide levels and increases post-thaw human sperm viability

doi: 10.1017/s0967199417000600

Figure Lengend Snippet: Figure 6 Post-thaw analyses of sperm exposed to L-CCMP, I-CCMP, or H-CCMP and storage in an ultrafreezer at −80°C. (a) Live cells were determined by flow cytometry using PI. Cell viability was measured by cfDNA using PicoGreen® assay (b), as well as by crystal violet assay (c). Levels of ROS (d), NO (e), and mitochondrial function (f) were measured by DCFC-DA, Griss reaction, and MTT assay, respectively. The results were compared with the control (cells and medium only). N = 3, ∗∗P < 0.001.

Article Snippet: The Quant-ITTM PicoGreen® double-stranded DNA kit (Invitrogen, Life Technologies) was used to perform cfDNA determination.

Techniques: Flow Cytometry, Picogreen Assay, Crystal Violet Assay, MTT Assay, Control

Journal: Cell Transplantation

Article Title: BMP2 is Superior to BMP4 for Promoting Human Muscle-Derived Stem Cell-Mediated Bone Regeneration in a Critical-Sized Calvarial Defect Model

doi: 10.3727/096368912x658854

Figure Lengend Snippet: Figure 4. The effect of BMP4 protein stimulation on hMDSC proliferation in vitro. (A) hMDSC proliferation increased after 3 days when 50 or 100 ng/ml of BMP4 was added to the culture medium on day 1. (B) hMDSC proliferation decreased after 5 days when BMP4 was added on days 1 and 3 using three different dosages (25, 50, or 100 ng/ml). *p < 0.05, **p < 0.01 compared to the untreated controls. n = 4 in each treatment group. (C) hMDSCs did not express alkaline phosphatase (AP) after BMP4 stimulation at 3 or 5 days. Scale bars: 200 µm. (D) C2C12 cells expressed AP after BMP4 stimulation for 3 days. Scale bars: 200 µm. dsDNA, double-stranded DNA.

Article Snippet: After stimulation, the cells were washed with PBS, and the DNA content was measured using a Quant-iTTM Picogreen double-stranded DNA (dsDNA) assay kit (Molecular Probes/Invitrogen).

Techniques: In Vitro